Anti-Mycobacterium tuberculosis, ESAT-6
Mouse monoclonal antibody
Cat.No. HYB 076-08
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
ESAT-6 (6-kDa early secretory antigenic target) from Mycobacterium tuberculosis H37Rv (ATCC 27294) (1) is a secreted tubercular protein that forms a dominant target for cell-mediated immunity in the early phase of infection (2). The deduced amino acid sequence indicates a molecular mass of 10 kDa. It is present in M. tuberculosis, virulent strains of M. bovis, and in M. flavescens, M. kansasii, M. marinum, and M. szulgai, but not in avirulent M. bovis BCG substrains (3).
Purified protein derivative (PPD) of tuberculin from Mycobacterium tuberculosis cojugatedwith thimerosal N-hydroxysuccinimide ester and adsorbed to aluminum hydroxide gel (4)
HYB 076-08 reacts with ESAT-6 in immunoblotting after SDS-PAGE of short-term culture filtrates of M. tuberculosis and virulent strains of M. bovis. HYB 076-08 cross-reacts with MPB64 (26 kDa) from certain BCG substrains, with the A, B and C components of the antigen 85 complex and with MPT51.
Binds to residues 3-15 (EQQWNFAGIEAAA) of ESAT-6 (5)
HYB 076-08 reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/3200 | 5 |
| Immunoblotting | Yes | 1,3,4 | |
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for PPD coated at 10 µg/mL. Users should determine the optimal dilutions for their own purposes.
1. Sørensen AL, Nagai S, Houen G, Andersen P, Andersen AB (1995) Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect Immun 63:1710-17.
2. Ravn P, Demissie A, Eguale T, Wondwosson H, Lein D, Amoudy HA, Mustafa AS, Jensen AK, Holm A, Rosenkrands I, Oftung F, Olobo J, von Reyn F, Andersen P (1999) Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J Infect Dis 179:637-645.
3. Harboe M, Oettinger T, Wiker HG, Rosenkrands I, Andersen P (1996) Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infect Immun 64:16-22.
4. Klausen J, Magnusson M, Andersen AB, Koch C (1994) Characterization of purified protein derivative of tuberculin by use of monoclonal antibodies: isolation of a delayed-type hypersensitivity reactive component from M. tuberculosis culture filtrate. Scand J Immunol 40:345-349.
5. Harboe M, Malin AS, Dockrell HS, Wiker HG, Ulvund G, Holm A, Jorgensen MC, Andersen P (1998) B-Cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tuberculosis. Infect Immun 66:717-723.
